SOPs for CMD animal models

SOPs for CMD animal models
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Standard protocols

Cure CMD and TREAT-NMD are collaborating in establishing standard protocols for efficacy studies in the laminin deficient mouse model LAMA2dy-W, the best described mouse model for a CMD disease, and with the aid of AFM funding, also for additional CMD animal models.  With the involvement of several dedicated CMD researchers, readout parameters were selected that are appropriate to show the efficacy of a new potential drug, and that are related to patient outcomes. For each of these parameters, small groups of experts will develop a standard assessment protocol. The availability of standard protocols will implement research and increase the comparability of results.

The SOPs that have already been completed can be downloaded from this site and others will be added in the future. The last date each SOP was reviewed can be found under the code on the left of the page.

LAMA2dy-W Model

25 June 2016

Assessment of peripheral nerve pathology in laminin-alpha-2-deficient mice

Jeffrey B. Miller

28 January 2014

Quantitative determination of minimal Feret’s diameter, including the evaluation of the percentage of centralized nuclei, fiber numbers, cross-sectional area and the percentage of fibrosis

Markus A. Rüegg

28 January 2014

Histopathology in Masson Trichrome stained muscle sections

Markus A. Rüegg

28 January 2014

Histopathology in Hematoxylin & Eosin stained muscle sections

Markus A. Rüegg

25 January 2014

Inflammation quantification in the LAMA2dy-W mouse

Dean Burkin

26 July 2016

Neuromuscular junction imaging on whole muscles and muscle
sections in CMD animal models
Gregorius Valdez

25 January 2014

Recommendations on the care and handling of the MDC1A mouse model dyW

Janice Dominov

12 July 2017

Use of Grip Strength Meter to Assess the Limb Strength of dyw Mice Anne Connolly

23 May 2015

Single muscle contractile measurements in vivo and in situ Rich Lovering

12 July 2017

Serum Creatine Kinase analysis in mouse models of muscular dystrophy

Kanneboyina Nagaraju
8 April 2014

Behavioral Phenotyping for neonates: Righting reflex

C. DiDonato
L. Bogdanik

16 August 2017

Non-invasive measurement of ankle flexion/extension torque for
longitudinal follow-up of strength in small rodents

Jean-Yves Hogrel

10 July 2017

Gait assessment in a canine model of XLMTM using an instrumented carpet

Melissa Goddard
10 July 2017

Respiratory assessment of a canine model of XLMTM using non-invasive respiratory impedence plethysmography

Melissa Goddard

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Disclaimer: TREAT-NMD and the authors of the SOPs disclaim, without limitation, all liability for any loss or damage of any kind, including any direct, indirect or consequential damages, which might be incurred in connection with the information and procedures contained in these SOPs.

16 Aug 2017